The bulk kit comes with reagent 1 (1x 10ml) and reagent 2 (1x 1.7ml)
How much DNA will expect to get?
With the Arcis DNA Sample Prep kit its expected that with a 30ul blood sample a typical concentration of 2-5ng/ul of DNA will be obtained. To increase DNA concentrations, up to 90ul of blood can be added to reagent one. Ideally a ratio of 1:4 needs to be maintained
How should I quantitate DNA using the Arcis system?
For quantification of DNA the most accurate method is standard-curve qPCR. The Arcis system does not isolate DNA from carryover cellular-contents so spectrophotometric approaches, such as nanodrop, are not recommended. Fluorometric approaches which specifically bind DNA, such as the Qubit system, may be more reliable
How should I store the Arcis DNA Sample Prep Kits?
They are shipped and stored at room temperature
How long is DNA stable in tube 1?
Our data indicates that DNA is stable in tube 1 for up to 30 days at ambient temperatures
How should the the bulk reagent be stored and handled?
Tubes are shipped and stored at room temperature. The bulk kit format has been designed for high-throughput genomic applications. We advise that the buffers are not exposed to the environment for extended periods and/or continual short exposures, if this occurs then unused buffer not used within 48 hours after opening should be disposed of. If required the buffers can be pre-aliquoted and then stored for later use
Arcis Biotechnology awarded funding under Innovate UK’s Biocatalyst programme
Arcis Chief Scientist, Jan Rogers, meets PM Theresa May for business leaders discussion on industrial strategy
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