Frequently Asked Questions

  • What are the volumes of tube 1 and tube 2?
    • The bulk kit comes with reagent 1 (1x 10ml) and reagent 2 (1x 1.7ml)
  • How much DNA will expect to get?
    • With the Arcis DNA Sample Prep kit its expected that with a 30ul blood sample a typical concentration of 2-5ng/ul of DNA will be obtained. To increase DNA concentrations, up to 90ul of blood can be added to reagent one. Ideally a ratio of 1:4 needs to be maintained
  • How should I quantitate DNA using the Arcis system?
    • For quantification of DNA the most accurate method is standard-curve qPCR. The Arcis system does not isolate DNA from carryover cellular-contents so spectrophotometric approaches, such as nanodrop, are not recommended. Fluorometric approaches which specifically bind DNA, such as the Qubit system, may be more reliable
  • How should I store the Arcis DNA Sample Prep Kits?
    • They are shipped and stored at room temperature
  • How long is DNA stable in tube 1?
    • Our data indicates that DNA is stable in tube 1 for up to 30 days at ambient temperatures
  • How should the the bulk reagent be stored and handled?
    • Tubes are shipped and stored at room temperature. The bulk kit format has been designed for high-throughput genomic applications. We advise that the buffers are not exposed to the environment for extended periods and/or continual short exposures, if this occurs then unused buffer not used within 48 hours after opening should be disposed of. If required the buffers can be pre-aliquoted and then stored for later use