Arcis sample prep technology is a fast and convenient 2-step protocol for preparation of nucleic acids from a variety of sample types. The nucleic acids are prepared and stabilised in 3 minutes without the need for instrumentation or complicated heating or cooling steps. The DNA and RNA extracted is suitable for direct use in PCR, qPCR and RT-qPCR reactions using standard and fast cycling protocols.
The Arcis Sample Prep system consists of 2-steps. In the first step the sample is combined with a chemical which results in powerful and controlled lysis of cells and tissue. The first step also simultaneously chelates the nucleic acid and stabilises DNA for up to 90 days at room temperature. The critical second step contains a proprietary buffer which removes the nucleic acid chelation and relaxes the DNA/RNA in preparation for PCR or RT-qPCR. The proprietary buffer has also been formulated to mop up any PCR inhibitors that may have been carried over from the first step.
Rapid DNA extraction for bacterial cultures of Pseudomonas aeruginosa
Case study: A comparison of Arcis Sample Prep with 3 commercial DNA extraction kits Fothergill J and Hilliam Y, University of Liverpool (2015)
The Arcis Sample Prep kits were compared to market leading kits and evaluated for speed and yield. The Arcis DNA Sample Prep Kits required no instrumentation and took less than three minutes from start to finish. In contrast all the other kits required centrifugation or heating and frequently required a separate lysis step before the protocol. The processing times of the kits ranged from 20 minutes to 4 hours. The total DNA extracted from each kit was assessed using the Qubit System. The P kit yielded the most DNA, but was labour intensive and time consuming kit. The Arcis kit was comparable to the Q kit, however DNA was obtained in 3 minutes compared to 60 minutes for the Q system.
A range of forensics-relevant samples were processed using the Arcis Sample Prep Kits. Total processing time for each sample was 3 minutes. A range of samples were tested including buccal swabs, hair follicles, and dried blood on card or swabbed from a surface. Strong amplification curves were generated for each sample tested. For whole blood, very strong signals were generated from as little as 0.2μl starting material.Download
Arcis reagents can dramatically accelerate pre-analytical sample prep without compromising the quantity and quality of genomic material. Arcis Sample Prep Kits are supplied as a ready-to-use kit for application on a wide range of sample types including whole blood, plasma, urine, buccal swabs, bacteria and parasites. The DNA released is immediately suitable for downstream molecular investigations.Download