Arcis Sample Prep Technology

Arcis sample prep technology is a fast and convenient 2-step protocol for preparation of nucleic acids from a variety of sample types. The nucleic acids are prepared and stabilised in 3 minutes without the need for instrumentation or complicated heating or cooling steps. The DNA and RNA extracted is suitable for direct use in PCR, qPCR and RT-qPCR reactions using standard and fast cycling protocols.

  • Simple 2-step process
  • No instrumentation required
  • Nucleic acids stabilised after 1 minute – DNA shown to be stable in Reagent 1 for up to 90 days
  • Shipped and stored at ambient temperature

Step 1

Add sample

Reagent 1:

Opens up cells and releases nucleic acids in less than 1 minute. At this point the nucleic acids are released and protected by the Arcis solution, but are not in a usable state. DNA in Reagent 1 is stable for up to 90 days at room temperature.

Step 2

Add sample

Reagent 2:

Binds inhibitors and enhances PCR while also relaxing the DNA into a usable confirmation.  At this point the sample should be used immediately, going straight into PCR, qPCR, RT-qPCR or sequencing.

Relaxation of DNA

The Arcis Sample Prep system consists of 2-steps. In the first step the sample is combined with a chemical which results in powerful and controlled lysis of cells and tissue. The first step also simultaneously chelates the nucleic acid and stabilises DNA for up to 90 days at room temperature. The critical second step contains a proprietary buffer which removes the nucleic acid chelation and relaxes the DNA/RNA in preparation for PCR or RT-qPCR. The proprietary buffer has also been formulated to mop up any PCR inhibitors that may have been carried over from the first step.

Validation data

Rapid DNA extraction for bacterial cultures of Pseudomonas aeruginosa

Case study: A comparison of Arcis Sample Prep with 3 commercial DNA extraction kits Fothergill J and Hilliam Y, University of Liverpool (2015)

The comparator kits all require instrumentation such as centrifugation and heating, the processing times range from 20 minutes to 4 hours. The Arcis DNA Sample Prep Kits require no instrumentation and take less than three minutes from start to finish. The total DNA extracted from each kit was assessed using the Qubit System. The Promega kit yielded the most DNA however results were found to be highly variable. Large amounts of DNA was extracted from some of the isolates but this was not consistent. This is also the most labour intensive and time consuming kit. The Arcis kit was comparable to the Qiagen kit but DNA is obtained in 3 minutes compared to 60 minutes for the Qiagen system.

Forensic

A range of forensics-relevant samples were processed using the Arcis Sample Prep Kits. Total processing time for each sample was 3 minutes. A range of samples were tested including buccal swabs, hair follicles, and dried blood on card or swabbed from a surface. Strong amplification curves were generated for each sample tested. For whole blood, very strong signals were generated from as little as 0.2μl starting material.

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Generic

Arcis reagents can dramatically accelerate pre-analytical sample prep without compromising the quantity and quality of genomic material. Arcis Sample Prep Kits are supplied as a ready-to-use kit for application on a wide range of sample types including whole blood, plasma, urine, buccal swabs, bacteria and parasites. The DNA released is immediately suitable for downstream molecular investigations.

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