Arcis DNA Sample Prep Technology

Arcis sample prep technology is a fast and convenient 2-step protocol for preparation of nucleic acid from a variety of sample types. DNA is prepared and stabilised in 3 minutes without the need for instrumentation or complicated heating or cooling steps. The DNA is suitable for use in PCR and qPCR reactions using standard and fast cycling protocols.

  • Simple 2-step process
  • No instrumentation required
  • DNA stable for 2 weeks
  • Shipped and stored at ambient temperature

Step 1

Add sample

Tube 1:

Opens up cells and releases nucleic acids in less than 1 minute. At this point the DNA is released and protected by the Arcis solution, but is not in a usable state. DNA will be stable for 2 weeks.

Step 2

Add sample

Tube 2:

Binds inhibitors and enhances PCR while also relaxing the DNA into a usable confirmation.

Relaxation of DNA

The Arcis DNA Sample Prep system consists of 2-steps. In the first step the sample is combined with a chemical which results in powerful and controlled lysis of cells and tissue. The first step also simultaneously chelates the nucleic acid and stabilises the DNA/RNA for at least 30 days at room temperature. The critical second step contains a propitiatory buffer which removes the nucleic acid chelation and relaxes the DNA/RNA in preparation for PCR. The proprietary buffer has also been formulated to mop up any PCR inhibitors that may have been carried over from the first step.

Validation data

Rapid DNA extraction for bacterial cultures of Pseudomonas aeruginosa

Case study: A comparison of PCRdirect® Ultra-Fast Sample Prep with 3 commercial DNA extraction kits Fothergill J and Hilliam Y, University of Liverpool (2015)

The comparator kits all require instrumentation such as centrifugation and heating, the processing times range from 20 minutes to 4 hours. The Arcis DNA Sample Prep Kits require no instrumentation and take less than three minutes from start to finish. The total DNA extracted from each kit was assessed using the Qubit System. The Promega kit yielded the most DNA however results were found to be highly variable. Large amounts of DNA was extracted from some of the isolates but this was not consistent. This is also the most labour intensive and time consuming kit. The Arcis kit was comparable to the Qiagen kit but DNA is obtained in 3 minutes compared to 60 minutes for the Qiagen system.

Forensic

A range of forensics-relevant samples were processed using the Arcis DNA Sample Prep Kits. Total processing time for each sample was 3 minutes. A range of samples were tested including buccal swabs, hair follicles, and dried blood on card or swabbed from a surface. Strong amplification curves were generated for each sample tested. For whole blood, very strong signals were generated from as little as 0.2μl starting material.

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Arcis reagents can dramatically accelerate pre-analytical sample prep without compromising the quantity and quality of genomic material. Arcis DNA Sample Prep Kits are supplied as a ready-to-use kit for application on a wide range of sample types including whole blood, plasma, urine, buccal swabs, bacteria and parasites. The DNA released is immediately suitable for downstream molecular investigations.

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